1. Understanding the desired concentration of the solution, which is typically expressed in molarity (moles of solute per liter of solution).
2. Calculating the amount of solute needed to achieve the desired concentration, using the formula: moles = molarity × volume.
3. Accurately weighing the solute using a laboratory balance, making sure to tare the container before adding the solute.
4. Dissolving the solute in a suitable solvent, such as distilled water or a buffer solution, by adding the solvent to the solute and stirring or vortexing until the solute is completely dissolved.
5. Transferring the dissolved solute to a volumetric flask of the appropriate size, ensuring that the final volume of the solution matches the desired volume.
6. Adding more solvent to the volumetric flask until the solution reaches the calibration mark on the flask.
7. Thoroughly mixing the solution by inverting the flask several times or stirring with a glass rod.
8. Labeling the flask with the solution's concentration, the date it was prepared, and any other relevant information.